|
This experiment was performed to examine the possibility of utilizing peroxidase activity as a biochemical marker for organogenic ability of Gladiolus callus. Calli derived from cormel tissues of ´Nova Lux´, ´White Prosperity´, and ´White Friendship´ turned brown and showed highest peroxidase activity. The calli of ´Spic & Span´, ´True Love´, ´Red Beauty´ and ´Hunting Song´ regenerated spontaneously during subculture and it was difficult to maintain the cultures as callus, whereas the ´Topaz´ callus was lowest in peroxidase activity and could be maintained and proliferated. The peroxidase activities of ´Topaz´ calli subcultured with different kinds of auxin were compared. Peroxidase activities in the callus subcultured on the medium containing 0.5 ㎎/L NAA were higher than those on the medium containing 0.5 ㎎/L 2,4-D. The former formed organs during subculture, but the latter proliferated callus only. Protein contents and peroxidase activities of non-organogenic callus (NOGC) were much lower than those of organogenic callus (OGC). The pattern of peroxidase isozyme was analyzed for root, leaf, corm, OGC and NOGC of Gladious ´Topaz´ by native PAGE. Ten isozyme bands of peroxidase were found in Gladiolus ´Topaz . NOGC showed less amount and number of peroxidase isozyme. With these results, we concluded that peroxidase is an useful biochemical marker to indicate organogenic competence Gladiolus callus in mass culture.
|